The principle of FPLC technology is the same as that of HPLC, that is, under high pressure (20-200 kg/cm2), the difference in migration rate of the separated substances in the liquid carrier is used for separation and purification.
When using HPLC to separate and purify biological macromolecules, because the liquid carrier is usually an organic solvent, it is easy to denature and inactivate biological macromolecules, which reduces the recovery rate of biological macromolecules. At the same time, non inert stainless steel is used to manufacture the flow circuit of the HPLC system, and metal ions may dissociate under the working state, thus adsorbing some protein components.
The chromatographic column of FPLC is a high pressure resistant organic glass tube. Other tubes are made of high pressure resistant non-metallic materials. At the same time, inert liquid carrier is used to enable safe and rapid separation of protein components. Therefore, FPLC retains the characteristics of simple operation, strong repeatability and fast separation of HPLC, and has higher resolution and practicability in the separation and purification of biological macromolecules. FPLC has its own special pre packed column, and various chromatographic columns such as molecular sieve, ion exchange, hydrophobic chromatography are also equipped, which are widely used in biology, chemistry, environmental science and other fields.
Protein Purification System | Inscinstech
Inscinstech’s protein purification system is mainly used in biopharmaceutical fields such as monoclonal antibodies, recombinant proteins, vaccines, biochemical drugs, antibiotics, natural products and polysaccharides. It is used for the separation and purification of downstream processes of biological products.
l Flow rate range: 0.001-600 ml/min
l Automatic chromatography system
l Matched with automatic sampler and collector
From laboratory process development to GMP production