The full name of FPLC is Fast Protein Liquid Chromatography. Its principle is similar to the theory of high performance liquid chromatography. It is a modern liquid chromatography developed by introducing the gas chromatography theory from the classic liquid column chromatography and reforming the phase, using high pressure infusion pumps, highly sensitive detectors, gradient elution devices, automatic collection devices and microcomputers.
FPLC is a system specially used to separate proteins, polypeptides and polynucleotides. It is an important innovation of HPLC in recent years. It not only maintains the fast and high resolution characteristics of HPLC, but also has the characteristics of large column capacity, high recovery efficiency and not easy to inactivate biological macromolecules.
With the development of HPLC, two new types of packing materials have emerged: thin shell packing and double diffusion perfusion packing. They have unique advantages in the separation of macromolecular substances, thus expanding the application field of FPLC. It can be applied to the separation and purification of proteins and organic compounds, and can quantitatively determine the content of proteins and determine the molecular weight of proteins. Therefore, it can be applied to the research in biological and chemical fields.
FPLC Application Scope
FPLC is specially used to separate and purify proteins, polypeptides and polynucleotides.
HPLC can analyze low boiling point and low relative molecular weight molecules, high boiling point middle molecules, macromolecular organic compounds (including polar and non-polar), ionic inorganic compounds, and thermally unstable biomolecules.
Protein Purification System | Inscinstech
Inscinstech’s protein purification system is mainly used in biopharmaceutical fields such as monoclonal antibodies, recombinant proteins, vaccines, biochemical drugs, antibiotics, natural products and polysaccharides. It is used for the separation and purification of downstream processes of biological products.
l Flow rate range: 0.001-600 ml/min
l Automatic chromatography system
l Matched with automatic sampler and collector
l From laboratory process development to GMP production