Encyclopedia of Protein Purification System


About protein purification system

There are many proteins in cells. Protein separation and purification is to extract some proteins from cells, which is an indispensable link in protein research.

Protein separation and purification can be widely used in pharmaceutical, outpatient laboratory, biochemistry, scientific research and other fields. This is an important operation technology.


-PART 1-

The significance of protein purification system

In order to study the essence of life, it is necessary to purify proteins from biological materials and study their structures and functions, which is of great significance for understanding the laws of life activities and clarifying the essence of life phenomena.

Industrial production needs food, fermentation, textile, leather and other industries need a large number of highly active enzyme preparations. For example, amylase is used to produce glucose, maltose, dextrin and syrup.

Many proteins needed for medical treatment are safe and effective drugs. For example, proteolytic enzyme complexes are widely used as digestive drugs, and urokinase can treat various thrombotic diseases.

Genetic engineering requires the modification of proteins and enzymes with physiological activity to improve their physiological activity, enzyme activity and protein thermal stability. The purified protein must be obtained first, and then modified on the basis of the pure protein, such as site directed mutation.



-PART 2-

Main methods of protein purification system

1. Separation methods according to different molecular sizes: dialysis and ultrafiltration (using the property that protein molecules cannot pass through the semi permeable membrane); Density gradient centrifugation (when protein is centrifuged in medium, particles with larger mass and density will settle faster); Gel filtration (a kind of column chromatography);

2. Separation by solubility difference: isoelectric point precipitation method (since the net charge of protein molecules at the isoelectric point is zero, which reduces the electrostatic repulsion between molecules, it is easy to accumulate and precipitate, and the solubility is minimum at this time); Salt dissolution and salting out (using a certain concentration of salt solution to increase or decrease the solubility of protein);

3. According to different charge separation methods, they mainly include electrophoresis and ion exchange chromatography;

4. Selective adsorption and separation of proteins (the purpose of separation is achieved by using the different adsorption strength of particles);

5. Separation according to the characteristics of ligands - affinity chromatography (using the biological property that protein molecules can specifically but not covalently combine with another molecule called ligand);

6. Low temperature organic solvent precipitation method: Most proteins can be dissolved and precipitated by using an organic solvent that is miscible with water, methanol, ethanol or acetone. This method has higher resolution than salting out, but the protein is easy to denature, so it should be carried out at low temperature.



-PART 3-

Common protein protein purification system

1. Gel chromatography medium

2. Protein affinity chromatography medium

3. Other affinity purification media

4. Ion exchange chromatography medium

5. Hydrophobic chromatography medium

6. Magnetic microsphere

7. Composite mode chromatography medium


Protein Purification System | Inscinstech

Inscinstechs protein purification system is mainly used in biopharmaceutical fields such as monoclonal antibodies, recombinant proteins, vaccines, biochemical drugs, antibiotics, natural products and polysaccharides. It is used for the separation and purification of downstream processes of biological products.



Flow rate range: 0.001-600 ml/min

Automatic chromatography system

Matched with automatic sampler and collector

From laboratory process development to GMP production