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How are oligos synthesized?

How are oligos synthesized?

For large scale oligonucleotide synthesis, the Phosphoramidite method is the most widely used method, which is a kind of solid phase synthesis

2022-7-14
What is the difference between nucleotide and oligonucleotide?

What is the difference between nucleotide and oligonucleotide?

Nucleotide is (biochemistry) the monomer comprising DNA or RNA, while oligonucleotide is a short sequence of nucleotides (rna or dna), typically with twenty or fewer base pairs.

2022-7-14
What is the difference between Poly and oligo?

What is the difference between Poly and oligo?

Oligonucleotides are short nucleic acid polymers which are usually made up of 13 to 25 nucleotides and are designed to hybridize specifically to DNA or RNA sequences, while poly usually refers to larger ones.

2022-7-14
What To Consider When Choosing Chromatography Equipment?

What To Consider When Choosing Chromatography Equipment?

Scale/flowrate, automation, pressure resistence, sensors, easy to use software, service, etc.

2022-7-14
What is the difference between HPLC and FPLC?

What is the difference between HPLC and FPLC?

FPLC stands for fast protein liquid chromatography and HPLC stands for high performance liquid chromatography.

2022-7-14